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If creating v2 sample sheets for devices in addition to the ones detailed, affirm the ultimate sample sheet has the i5 index in the proper orientation as described over.
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A quick, integrated workflow for planning libraries for use in a wide array of sequencing applications.
Make entire-genome sequencing libraries and competently interrogate samples with constrained available DNA.
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When making use of BaseSpace Sequence Hub PrepTab, i5 sequences should be entered into these software program in the ahead orientation more info in all conditions and for all instrument varieties.
The sequences exhibited are what on earth is entered into the final sample sheet. Kit definitions contain the i5 while in the forward orientation. The i5 sequences will be correctly reverse complemented and shown in the IEM interface dependant on instrument/reagent form.